Forsyth Bioinformatics

BLAST-Based Open-Reference 16S sRNA NGS Species-Level Read Assignment Pipeline With QIIME Results Code Category Minimal read count per species (MC) (Click for detail report) MC1 (All) MC10 MC100 A Total reads 611,225 611,225 611,225 B Total samples 40 40 40 C Samples with reads ≥ 3000 reads (S≥3000) 40 40 40 D Samples with reads < 3000 reads (S<3000) 0 0 0 E Reads in samples S≥3000 611,225 611,225 611,225 F Reads in samples S<3000 (removed) 0 0 0 G Total reads assigned 574,296 572,289 562,037 H Reads assigned to single species 542,535 541,711 534,534 I Reads assigned to multiple species 7,982 7,875 7,402 J Reads assigned to novel species 23,779 22,703 20,101 K Number of single species 661 425 236 L Number of multi-species 44 20 9 M Number of novel species 421 114 26 N Total reads unassigned 36,929 38,936 49,188 O Reads excluded (<MC) 0 2,007 12,259 P Chimeric reads 172 172 172 Q Reads without BLASTN hits 184 184 184 R Singleton reads 36,573 36,573 36,573 1. A=E+F A=F+G+N G=H+I+J N=O+P+Q+R 2. View all read count by samples 3. All data can be downloaded in a compressed ZIP file ZIP file 4. All data are also available on the FTP site for 30 days 5. Please read DOCUMENTATION (pdf) 6. Preferred web browser: Google Chrome or FireFox, especially for 2D and 3D plots 7. Contact: George Chen, Email: tchen\@forsyth.org for questions