Forsyth Bioinformatics

BLAST-Based Open-Reference 16S sRNA NGS Species-Level Read Assignment Pipeline With QIIME Results Code Category Minimal read count per species (MC) (Click for detail report) MC1 (All) MC10 MC100 A Total reads 442,101 442,101 442,101 B Total samples 48 48 48 C Samples with reads ≥ 3000 reads (S≥3000) 48 48 48 D Samples with reads < 3000 reads (S<3000) 0 0 0 E Reads in samples S≥3000 442,101 442,101 442,101 F Reads in samples S<3000 (removed) 0 0 0 G Total reads assigned 394,937 392,889 382,211 H Reads assigned to single species 383,165 382,255 374,221 I Reads assigned to multiple species 2,445 2,397 1,930 J Reads assigned to novel species 9,327 8,237 6,060 K Number of single species 675 414 193 L Number of multi-species 32 20 4 M Number of novel species 373 98 12 N Total reads unassigned 47,164 49,212 59,890 O Reads excluded (<MC) 0 2,048 12,726 P Chimeric reads 65 65 65 Q Reads without BLASTN hits 61 61 61 R Singleton reads 47,038 47,038 47,038 1. A=E+F A=F+G+N G=H+I+J N=O+P+Q+R 2. View all read count by samples 3. All data can be downloaded in a compressed ZIP file ZIP file 4. All data are also available on the FTP site for 30 days 5. Please read DOCUMENTATION (pdf) 6. Preferred web browser: Google Chrome or FireFox, especially for 2D and 3D plots 7. Contact: George Chen, Email: tchen\@forsyth.org for questions