Logging started at 17:42:22 on 12 Oct 2016 QIIME version: 1.9.1 qiime_config values: blastmat_dir /qiime_software/blast-2.2.22-release/data pick_otus_reference_seqs_fp /usr/local/lib/python2.7/dist-packages/qiime_default_reference/gg_13_8_otus/rep_set/97_otus.fasta sc_queue all.q pynast_template_alignment_fp /usr/local/lib/python2.7/dist-packages/qiime_default_reference/gg_13_8_otus/rep_set_aligned/85_otus.pynast.fasta cluster_jobs_fp start_parallel_jobs.py assign_taxonomy_reference_seqs_fp /usr/local/lib/python2.7/dist-packages/qiime_default_reference/gg_13_8_otus/rep_set/97_otus.fasta torque_queue friendlyq jobs_to_start 1 denoiser_min_per_core 50 assign_taxonomy_id_to_taxonomy_fp /usr/local/lib/python2.7/dist-packages/qiime_default_reference/gg_13_8_otus/taxonomy/97_otu_taxonomy.txt temp_dir /tmp/ blastall_fp /qiime_software/blast-2.2.22-release/bin/blastall seconds_to_sleep 1 parameter file values: alpha_diversity:metrics berger_parker_d,brillouin_d,chao1,chao1_ci,dominance,doubles,enspie,equitability,esty_ci,fisher_alpha,gini_index,goods_coverage,heip_e,kempton_taylor_q,margalef,mcintosh_d,mcintosh_e,menhinick,observed_otus,observed_species,osd,simpson_reciprocal,robbins,shannon,simpson,simpson_e,singles,strong,PD_whole_tree beta_diversity:metrics abund_jaccard,binary_chisq,binary_chord,binary_euclidean,binary_hamming,binary_jaccard,binary_lennon,binary_ochiai,binary_pearson,binary_sorensen_dice,bray_curtis,bray_curtis_faith,bray_curtis_magurran,canberra,chisq,chord,euclidean,gower,hellinger,kulczynski,manhattan,morisita_horn,pearson,soergel,spearman_approx,specprof,unifrac,unweighted_unifrac,unweighted_unifrac_full_tree,weighted_normalized_unifrac,weighted_unifrac summarize_taxa:level 2,3,4,5,6,7 parallel:jobs_to_start 1 filter_samples_from_otu_table:min_count 500 plot_taxa_summary:labels Phylum,Class,Order,Family,Genus,Species plot_taxa_summary:chart_type bar,area,pie core_diversity_analyses:categories Toothpaste Input file md5 sums: qiime_results/data_mc100/final_otu_map_mc100_tax.biom: 7e5b682157327cf0756181bbf7eccd67 meta.txt: d8dc9993bcfe520a325021d8a9c0c21c qiime_results/data_mc100/rep_set_mc100.tre: 4076c304375b92a6e44008d267e9ac3b Executing commands. # Generate BIOM table summary command biom summarize-table -i qiime_results/data_mc100/final_otu_map_mc100_tax.biom -o qiime_results/cd_mc100/biom_table_summary.txt Stdout: Stderr: # Filter low sequence count samples from table (minimum sequence count: 24476) command filter_samples_from_otu_table.py -i qiime_results/data_mc100/final_otu_map_mc100_tax.biom -o qiime_results/cd_mc100/table_mc24476.biom -n 24476 Stdout: Stderr: # Rarify the OTU table to 24476 sequences/sample command single_rarefaction.py -i qiime_results/cd_mc100/table_mc24476.biom -o qiime_results/cd_mc100/table_even24476.biom -d 24476 Stdout: Stderr: Executing commands. # Beta Diversity (abund_jaccard) command beta_diversity.py -i qiime_results/cd_mc100/table_even24476.biom -o qiime_results/cd_mc100/bdiv_even24476/ --metrics abund_jaccard -t qiime_results/data_mc100/rep_set_mc100.tre Stdout: Stderr: /usr/local/lib/python2.7/dist-packages/numpy/core/fromnumeric.py:2507: VisibleDeprecationWarning: `rank` is deprecated; use the `ndim` attribute or function instead. To find the rank of a matrix see `numpy.linalg.matrix_rank`. VisibleDeprecationWarning) # Rename distance matrix (abund_jaccard) command mv qiime_results/cd_mc100/bdiv_even24476//abund_jaccard_table_even24476.txt qiime_results/cd_mc100/bdiv_even24476//abund_jaccard_dm.txt Stdout: Stderr: # Principal coordinates (abund_jaccard) command principal_coordinates.py -i qiime_results/cd_mc100/bdiv_even24476//abund_jaccard_dm.txt -o qiime_results/cd_mc100/bdiv_even24476//abund_jaccard_pc.txt Stdout: Stderr: /usr/local/lib/python2.7/dist-packages/skbio/stats/ordination/_principal_coordinate_analysis.py:107: RuntimeWarning: The result contains negative eigenvalues. Please compare their magnitude with the magnitude of some of the largest positive eigenvalues. If the negative ones are smaller, it's probably safe to ignore them, but if they are large in magnitude, the results won't be useful. See the Notes section for more details. The smallest eigenvalue is -1.43844675754 and the largest is 4.48049582385. RuntimeWarning # Make emperor plots, abund_jaccard) command make_emperor.py -i qiime_results/cd_mc100/bdiv_even24476//abund_jaccard_pc.txt -o qiime_results/cd_mc100/bdiv_even24476//abund_jaccard_emperor_pcoa_plot/ -m meta.txt Stdout: Stderr: # Beta Diversity (binary_chisq) command beta_diversity.py -i qiime_results/cd_mc100/table_even24476.biom -o qiime_results/cd_mc100/bdiv_even24476/ --metrics binary_chisq -t qiime_results/data_mc100/rep_set_mc100.tre Stdout: Stderr: /usr/local/lib/python2.7/dist-packages/numpy/core/fromnumeric.py:2507: VisibleDeprecationWarning: `rank` is deprecated; use the `ndim` attribute or function instead. To find the rank of a matrix see `numpy.linalg.matrix_rank`. VisibleDeprecationWarning) # Rename distance matrix (binary_chisq) command mv qiime_results/cd_mc100/bdiv_even24476//binary_chisq_table_even24476.txt qiime_results/cd_mc100/bdiv_even24476//binary_chisq_dm.txt Stdout: Stderr: # Principal coordinates (binary_chisq) command principal_coordinates.py -i qiime_results/cd_mc100/bdiv_even24476//binary_chisq_dm.txt -o qiime_results/cd_mc100/bdiv_even24476//binary_chisq_pc.txt Stdout: Stderr: # Make emperor plots, binary_chisq) command make_emperor.py -i qiime_results/cd_mc100/bdiv_even24476//binary_chisq_pc.txt -o qiime_results/cd_mc100/bdiv_even24476//binary_chisq_emperor_pcoa_plot/ -m meta.txt Stdout: Stderr: # Beta Diversity (binary_chord) command beta_diversity.py -i qiime_results/cd_mc100/table_even24476.biom -o qiime_results/cd_mc100/bdiv_even24476/ --metrics binary_chord -t qiime_results/data_mc100/rep_set_mc100.tre Stdout: Stderr: /usr/local/lib/python2.7/dist-packages/numpy/core/fromnumeric.py:2507: VisibleDeprecationWarning: `rank` is deprecated; use the `ndim` attribute or function instead. To find the rank of a matrix see `numpy.linalg.matrix_rank`. VisibleDeprecationWarning) # Rename distance matrix (binary_chord) command mv qiime_results/cd_mc100/bdiv_even24476//binary_chord_table_even24476.txt qiime_results/cd_mc100/bdiv_even24476//binary_chord_dm.txt Stdout: Stderr: # Principal coordinates (binary_chord) command principal_coordinates.py -i qiime_results/cd_mc100/bdiv_even24476//binary_chord_dm.txt -o qiime_results/cd_mc100/bdiv_even24476//binary_chord_pc.txt Stdout: Stderr: # Make emperor plots, binary_chord) command make_emperor.py -i qiime_results/cd_mc100/bdiv_even24476//binary_chord_pc.txt -o qiime_results/cd_mc100/bdiv_even24476//binary_chord_emperor_pcoa_plot/ -m meta.txt Stdout: Stderr: # Beta Diversity (binary_euclidean) command beta_diversity.py -i qiime_results/cd_mc100/table_even24476.biom -o qiime_results/cd_mc100/bdiv_even24476/ --metrics binary_euclidean -t qiime_results/data_mc100/rep_set_mc100.tre Stdout: Stderr: /usr/local/lib/python2.7/dist-packages/numpy/core/fromnumeric.py:2507: VisibleDeprecationWarning: `rank` is deprecated; use the `ndim` attribute or function instead. To find the rank of a matrix see `numpy.linalg.matrix_rank`. VisibleDeprecationWarning) # Rename distance matrix (binary_euclidean) command mv qiime_results/cd_mc100/bdiv_even24476//binary_euclidean_table_even24476.txt qiime_results/cd_mc100/bdiv_even24476//binary_euclidean_dm.txt Stdout: Stderr: # Principal coordinates (binary_euclidean) command principal_coordinates.py -i qiime_results/cd_mc100/bdiv_even24476//binary_euclidean_dm.txt -o qiime_results/cd_mc100/bdiv_even24476//binary_euclidean_pc.txt Stdout: Stderr: # Make emperor plots, binary_euclidean) command make_emperor.py -i qiime_results/cd_mc100/bdiv_even24476//binary_euclidean_pc.txt -o qiime_results/cd_mc100/bdiv_even24476//binary_euclidean_emperor_pcoa_plot/ -m meta.txt Stdout: Stderr: # Beta Diversity (binary_hamming) command beta_diversity.py -i qiime_results/cd_mc100/table_even24476.biom -o qiime_results/cd_mc100/bdiv_even24476/ --metrics binary_hamming -t qiime_results/data_mc100/rep_set_mc100.tre Stdout: Stderr: /usr/local/lib/python2.7/dist-packages/numpy/core/fromnumeric.py:2507: VisibleDeprecationWarning: `rank` is deprecated; use the `ndim` attribute or function instead. To find the rank of a matrix see `numpy.linalg.matrix_rank`. VisibleDeprecationWarning) # Rename distance matrix (binary_hamming) command mv qiime_results/cd_mc100/bdiv_even24476//binary_hamming_table_even24476.txt qiime_results/cd_mc100/bdiv_even24476//binary_hamming_dm.txt Stdout: Stderr: # Principal coordinates (binary_hamming) command principal_coordinates.py -i qiime_results/cd_mc100/bdiv_even24476//binary_hamming_dm.txt -o qiime_results/cd_mc100/bdiv_even24476//binary_hamming_pc.txt Stdout: Stderr: /usr/local/lib/python2.7/dist-packages/skbio/stats/ordination/_principal_coordinate_analysis.py:107: RuntimeWarning: The result contains negative eigenvalues. Please compare their magnitude with the magnitude of some of the largest positive eigenvalues. If the negative ones are smaller, it's probably safe to ignore them, but if they are large in magnitude, the results won't be useful. See the Notes section for more details. The smallest eigenvalue is -9239.39556077 and the largest is 336515.1551. RuntimeWarning # Make emperor plots, binary_hamming) command make_emperor.py -i qiime_results/cd_mc100/bdiv_even24476//binary_hamming_pc.txt -o qiime_results/cd_mc100/bdiv_even24476//binary_hamming_emperor_pcoa_plot/ -m meta.txt Stdout: Stderr: # Beta Diversity (binary_jaccard) command beta_diversity.py -i qiime_results/cd_mc100/table_even24476.biom -o qiime_results/cd_mc100/bdiv_even24476/ --metrics binary_jaccard -t qiime_results/data_mc100/rep_set_mc100.tre Stdout: Stderr: /usr/local/lib/python2.7/dist-packages/numpy/core/fromnumeric.py:2507: VisibleDeprecationWarning: `rank` is deprecated; use the `ndim` attribute or function instead. To find the rank of a matrix see `numpy.linalg.matrix_rank`. VisibleDeprecationWarning) # Rename distance matrix (binary_jaccard) command mv qiime_results/cd_mc100/bdiv_even24476//binary_jaccard_table_even24476.txt qiime_results/cd_mc100/bdiv_even24476//binary_jaccard_dm.txt Stdout: Stderr: # Principal coordinates (binary_jaccard) command principal_coordinates.py -i qiime_results/cd_mc100/bdiv_even24476//binary_jaccard_dm.txt -o qiime_results/cd_mc100/bdiv_even24476//binary_jaccard_pc.txt Stdout: Stderr: # Make emperor plots, binary_jaccard) command make_emperor.py -i qiime_results/cd_mc100/bdiv_even24476//binary_jaccard_pc.txt -o qiime_results/cd_mc100/bdiv_even24476//binary_jaccard_emperor_pcoa_plot/ -m meta.txt Stdout: Stderr: # Beta Diversity (binary_lennon) command beta_diversity.py -i qiime_results/cd_mc100/table_even24476.biom -o qiime_results/cd_mc100/bdiv_even24476/ --metrics binary_lennon -t qiime_results/data_mc100/rep_set_mc100.tre Stdout: Stderr: /usr/local/lib/python2.7/dist-packages/numpy/core/fromnumeric.py:2507: VisibleDeprecationWarning: `rank` is deprecated; use the `ndim` attribute or function instead. To find the rank of a matrix see `numpy.linalg.matrix_rank`. VisibleDeprecationWarning) # Rename distance matrix (binary_lennon) command mv qiime_results/cd_mc100/bdiv_even24476//binary_lennon_table_even24476.txt qiime_results/cd_mc100/bdiv_even24476//binary_lennon_dm.txt Stdout: Stderr: # Principal coordinates (binary_lennon) command principal_coordinates.py -i qiime_results/cd_mc100/bdiv_even24476//binary_lennon_dm.txt -o qiime_results/cd_mc100/bdiv_even24476//binary_lennon_pc.txt Stdout: Stderr: /usr/local/lib/python2.7/dist-packages/skbio/stats/ordination/_principal_coordinate_analysis.py:107: RuntimeWarning: The result contains negative eigenvalues. Please compare their magnitude with the magnitude of some of the largest positive eigenvalues. If the negative ones are smaller, it's probably safe to ignore them, but if they are large in magnitude, the results won't be useful. See the Notes section for more details. The smallest eigenvalue is -0.844761273624 and the largest is 2.76120306467. RuntimeWarning # Make emperor plots, binary_lennon) command make_emperor.py -i qiime_results/cd_mc100/bdiv_even24476//binary_lennon_pc.txt -o qiime_results/cd_mc100/bdiv_even24476//binary_lennon_emperor_pcoa_plot/ -m meta.txt Stdout: Stderr: # Beta Diversity (binary_ochiai) command beta_diversity.py -i qiime_results/cd_mc100/table_even24476.biom -o qiime_results/cd_mc100/bdiv_even24476/ --metrics binary_ochiai -t qiime_results/data_mc100/rep_set_mc100.tre Stdout: Stderr: /usr/local/lib/python2.7/dist-packages/numpy/core/fromnumeric.py:2507: VisibleDeprecationWarning: `rank` is deprecated; use the `ndim` attribute or function instead. To find the rank of a matrix see `numpy.linalg.matrix_rank`. VisibleDeprecationWarning) # Rename distance matrix (binary_ochiai) command mv qiime_results/cd_mc100/bdiv_even24476//binary_ochiai_table_even24476.txt qiime_results/cd_mc100/bdiv_even24476//binary_ochiai_dm.txt Stdout: Stderr: # Principal coordinates (binary_ochiai) command principal_coordinates.py -i qiime_results/cd_mc100/bdiv_even24476//binary_ochiai_dm.txt -o qiime_results/cd_mc100/bdiv_even24476//binary_ochiai_pc.txt Stdout: Stderr: /usr/local/lib/python2.7/dist-packages/skbio/stats/ordination/_principal_coordinate_analysis.py:107: RuntimeWarning: The result contains negative eigenvalues. Please compare their magnitude with the magnitude of some of the largest positive eigenvalues. If the negative ones are smaller, it's probably safe to ignore them, but if they are large in magnitude, the results won't be useful. See the Notes section for more details. The smallest eigenvalue is -0.06630463409 and the largest is 2.63682103599. RuntimeWarning # Make emperor plots, binary_ochiai) command make_emperor.py -i qiime_results/cd_mc100/bdiv_even24476//binary_ochiai_pc.txt -o qiime_results/cd_mc100/bdiv_even24476//binary_ochiai_emperor_pcoa_plot/ -m meta.txt Stdout: Stderr: # Beta Diversity (binary_pearson) command beta_diversity.py -i qiime_results/cd_mc100/table_even24476.biom -o qiime_results/cd_mc100/bdiv_even24476/ --metrics binary_pearson -t qiime_results/data_mc100/rep_set_mc100.tre Stdout: Stderr: /usr/local/lib/python2.7/dist-packages/numpy/core/fromnumeric.py:2507: VisibleDeprecationWarning: `rank` is deprecated; use the `ndim` attribute or function instead. To find the rank of a matrix see `numpy.linalg.matrix_rank`. VisibleDeprecationWarning) # Rename distance matrix (binary_pearson) command mv qiime_results/cd_mc100/bdiv_even24476//binary_pearson_table_even24476.txt qiime_results/cd_mc100/bdiv_even24476//binary_pearson_dm.txt Stdout: Stderr: # Principal coordinates (binary_pearson) command principal_coordinates.py -i qiime_results/cd_mc100/bdiv_even24476//binary_pearson_dm.txt -o qiime_results/cd_mc100/bdiv_even24476//binary_pearson_pc.txt Stdout: Stderr: /usr/local/lib/python2.7/dist-packages/skbio/stats/ordination/_principal_coordinate_analysis.py:107: RuntimeWarning: The result contains negative eigenvalues. Please compare their magnitude with the magnitude of some of the largest positive eigenvalues. If the negative ones are smaller, it's probably safe to ignore them, but if they are large in magnitude, the results won't be useful. See the Notes section for more details. The smallest eigenvalue is -0.111479432109 and the largest is 4.65653963722. RuntimeWarning # Make emperor plots, binary_pearson) command make_emperor.py -i qiime_results/cd_mc100/bdiv_even24476//binary_pearson_pc.txt -o qiime_results/cd_mc100/bdiv_even24476//binary_pearson_emperor_pcoa_plot/ -m meta.txt Stdout: Stderr: # Beta Diversity (binary_sorensen_dice) command beta_diversity.py -i qiime_results/cd_mc100/table_even24476.biom -o qiime_results/cd_mc100/bdiv_even24476/ --metrics binary_sorensen_dice -t qiime_results/data_mc100/rep_set_mc100.tre Stdout: Stderr: /usr/local/lib/python2.7/dist-packages/numpy/core/fromnumeric.py:2507: VisibleDeprecationWarning: `rank` is deprecated; use the `ndim` attribute or function instead. To find the rank of a matrix see `numpy.linalg.matrix_rank`. VisibleDeprecationWarning) # Rename distance matrix (binary_sorensen_dice) command mv qiime_results/cd_mc100/bdiv_even24476//binary_sorensen_dice_table_even24476.txt qiime_results/cd_mc100/bdiv_even24476//binary_sorensen_dice_dm.txt Stdout: Stderr: # Principal coordinates (binary_sorensen_dice) command principal_coordinates.py -i qiime_results/cd_mc100/bdiv_even24476//binary_sorensen_dice_dm.txt -o qiime_results/cd_mc100/bdiv_even24476//binary_sorensen_dice_pc.txt Stdout: Stderr: /usr/local/lib/python2.7/dist-packages/skbio/stats/ordination/_principal_coordinate_analysis.py:107: RuntimeWarning: The result contains negative eigenvalues. Please compare their magnitude with the magnitude of some of the largest positive eigenvalues. If the negative ones are smaller, it's probably safe to ignore them, but if they are large in magnitude, the results won't be useful. See the Notes section for more details. The smallest eigenvalue is -0.0644793608631 and the largest is 2.5894748463. RuntimeWarning # Make emperor plots, binary_sorensen_dice) command make_emperor.py -i qiime_results/cd_mc100/bdiv_even24476//binary_sorensen_dice_pc.txt -o qiime_results/cd_mc100/bdiv_even24476//binary_sorensen_dice_emperor_pcoa_plot/ -m meta.txt Stdout: Stderr: # Beta Diversity (bray_curtis) command beta_diversity.py -i qiime_results/cd_mc100/table_even24476.biom -o qiime_results/cd_mc100/bdiv_even24476/ --metrics bray_curtis -t qiime_results/data_mc100/rep_set_mc100.tre Stdout: Stderr: /usr/local/lib/python2.7/dist-packages/numpy/core/fromnumeric.py:2507: VisibleDeprecationWarning: `rank` is deprecated; use the `ndim` attribute or function instead. To find the rank of a matrix see `numpy.linalg.matrix_rank`. VisibleDeprecationWarning) # Rename distance matrix (bray_curtis) command mv qiime_results/cd_mc100/bdiv_even24476//bray_curtis_table_even24476.txt qiime_results/cd_mc100/bdiv_even24476//bray_curtis_dm.txt Stdout: Stderr: # Principal coordinates (bray_curtis) command principal_coordinates.py -i qiime_results/cd_mc100/bdiv_even24476//bray_curtis_dm.txt -o qiime_results/cd_mc100/bdiv_even24476//bray_curtis_pc.txt Stdout: Stderr: /usr/local/lib/python2.7/dist-packages/skbio/stats/ordination/_principal_coordinate_analysis.py:107: RuntimeWarning: The result contains negative eigenvalues. Please compare their magnitude with the magnitude of some of the largest positive eigenvalues. If the negative ones are smaller, it's probably safe to ignore them, but if they are large in magnitude, the results won't be useful. See the Notes section for more details. The smallest eigenvalue is -0.0901120846473 and the largest is 3.41574508444. RuntimeWarning # Make emperor plots, bray_curtis) command make_emperor.py -i qiime_results/cd_mc100/bdiv_even24476//bray_curtis_pc.txt -o qiime_results/cd_mc100/bdiv_even24476//bray_curtis_emperor_pcoa_plot/ -m meta.txt Stdout: Stderr: # Beta Diversity (bray_curtis_faith) command beta_diversity.py -i qiime_results/cd_mc100/table_even24476.biom -o qiime_results/cd_mc100/bdiv_even24476/ --metrics bray_curtis_faith -t qiime_results/data_mc100/rep_set_mc100.tre Stdout: Stderr: /usr/local/lib/python2.7/dist-packages/numpy/core/fromnumeric.py:2507: VisibleDeprecationWarning: `rank` is deprecated; use the `ndim` attribute or function instead. To find the rank of a matrix see `numpy.linalg.matrix_rank`. VisibleDeprecationWarning) # Rename distance matrix (bray_curtis_faith) command mv qiime_results/cd_mc100/bdiv_even24476//bray_curtis_faith_table_even24476.txt qiime_results/cd_mc100/bdiv_even24476//bray_curtis_faith_dm.txt Stdout: Stderr: # Principal coordinates (bray_curtis_faith) command principal_coordinates.py -i qiime_results/cd_mc100/bdiv_even24476//bray_curtis_faith_dm.txt -o qiime_results/cd_mc100/bdiv_even24476//bray_curtis_faith_pc.txt Stdout: Stderr: /usr/local/lib/python2.7/dist-packages/skbio/stats/ordination/_principal_coordinate_analysis.py:107: RuntimeWarning: The result contains negative eigenvalues. Please compare their magnitude with the magnitude of some of the largest positive eigenvalues. If the negative ones are smaller, it's probably safe to ignore them, but if they are large in magnitude, the results won't be useful. See the Notes section for more details. The smallest eigenvalue is -0.0901120846473 and the largest is 3.41574508444. RuntimeWarning # Make emperor plots, bray_curtis_faith) command make_emperor.py -i qiime_results/cd_mc100/bdiv_even24476//bray_curtis_faith_pc.txt -o qiime_results/cd_mc100/bdiv_even24476//bray_curtis_faith_emperor_pcoa_plot/ -m meta.txt Stdout: Stderr: # Beta Diversity (bray_curtis_magurran) command beta_diversity.py -i qiime_results/cd_mc100/table_even24476.biom -o qiime_results/cd_mc100/bdiv_even24476/ --metrics bray_curtis_magurran -t qiime_results/data_mc100/rep_set_mc100.tre Stdout: Stderr: /usr/local/lib/python2.7/dist-packages/numpy/core/fromnumeric.py:2507: VisibleDeprecationWarning: `rank` is deprecated; use the `ndim` attribute or function instead. To find the rank of a matrix see `numpy.linalg.matrix_rank`. VisibleDeprecationWarning) # Rename distance matrix (bray_curtis_magurran) command mv qiime_results/cd_mc100/bdiv_even24476//bray_curtis_magurran_table_even24476.txt qiime_results/cd_mc100/bdiv_even24476//bray_curtis_magurran_dm.txt Stdout: Stderr: # Principal coordinates (bray_curtis_magurran) command principal_coordinates.py -i qiime_results/cd_mc100/bdiv_even24476//bray_curtis_magurran_dm.txt -o qiime_results/cd_mc100/bdiv_even24476//bray_curtis_magurran_pc.txt Stdout: Stderr: /usr/local/lib/python2.7/dist-packages/skbio/stats/ordination/_principal_coordinate_analysis.py:107: RuntimeWarning: The result contains negative eigenvalues. Please compare their magnitude with the magnitude of some of the largest positive eigenvalues. If the negative ones are smaller, it's probably safe to ignore them, but if they are large in magnitude, the results won't be useful. See the Notes section for more details. The smallest eigenvalue is -0.0901120846473 and the largest is 3.41574508444. RuntimeWarning # Make emperor plots, bray_curtis_magurran) command make_emperor.py -i qiime_results/cd_mc100/bdiv_even24476//bray_curtis_magurran_pc.txt -o qiime_results/cd_mc100/bdiv_even24476//bray_curtis_magurran_emperor_pcoa_plot/ -m meta.txt Stdout: Stderr: # Beta Diversity (canberra) command beta_diversity.py -i qiime_results/cd_mc100/table_even24476.biom -o qiime_results/cd_mc100/bdiv_even24476/ --metrics canberra -t qiime_results/data_mc100/rep_set_mc100.tre Stdout: Stderr: /usr/local/lib/python2.7/dist-packages/numpy/core/fromnumeric.py:2507: VisibleDeprecationWarning: `rank` is deprecated; use the `ndim` attribute or function instead. To find the rank of a matrix see `numpy.linalg.matrix_rank`. VisibleDeprecationWarning) # Rename distance matrix (canberra) command mv qiime_results/cd_mc100/bdiv_even24476//canberra_table_even24476.txt qiime_results/cd_mc100/bdiv_even24476//canberra_dm.txt Stdout: Stderr: # Principal coordinates (canberra) command principal_coordinates.py -i qiime_results/cd_mc100/bdiv_even24476//canberra_dm.txt -o qiime_results/cd_mc100/bdiv_even24476//canberra_pc.txt Stdout: Stderr: # Make emperor plots, canberra) command make_emperor.py -i qiime_results/cd_mc100/bdiv_even24476//canberra_pc.txt -o qiime_results/cd_mc100/bdiv_even24476//canberra_emperor_pcoa_plot/ -m meta.txt Stdout: Stderr: # Beta Diversity (chisq) command beta_diversity.py -i qiime_results/cd_mc100/table_even24476.biom -o qiime_results/cd_mc100/bdiv_even24476/ --metrics chisq -t qiime_results/data_mc100/rep_set_mc100.tre Stdout: Stderr: /usr/local/lib/python2.7/dist-packages/numpy/core/fromnumeric.py:2507: VisibleDeprecationWarning: `rank` is deprecated; use the `ndim` attribute or function instead. To find the rank of a matrix see `numpy.linalg.matrix_rank`. VisibleDeprecationWarning) # Rename distance matrix (chisq) command mv qiime_results/cd_mc100/bdiv_even24476//chisq_table_even24476.txt qiime_results/cd_mc100/bdiv_even24476//chisq_dm.txt Stdout: Stderr: # Principal coordinates (chisq) command principal_coordinates.py -i qiime_results/cd_mc100/bdiv_even24476//chisq_dm.txt -o qiime_results/cd_mc100/bdiv_even24476//chisq_pc.txt Stdout: Stderr: # Make emperor plots, chisq) command make_emperor.py -i qiime_results/cd_mc100/bdiv_even24476//chisq_pc.txt -o qiime_results/cd_mc100/bdiv_even24476//chisq_emperor_pcoa_plot/ -m meta.txt Stdout: Stderr: # Beta Diversity (chord) command beta_diversity.py -i qiime_results/cd_mc100/table_even24476.biom -o qiime_results/cd_mc100/bdiv_even24476/ --metrics chord -t qiime_results/data_mc100/rep_set_mc100.tre Stdout: Stderr: /usr/local/lib/python2.7/dist-packages/numpy/core/fromnumeric.py:2507: VisibleDeprecationWarning: `rank` is deprecated; use the `ndim` attribute or function instead. To find the rank of a matrix see `numpy.linalg.matrix_rank`. VisibleDeprecationWarning) # Rename distance matrix (chord) command mv qiime_results/cd_mc100/bdiv_even24476//chord_table_even24476.txt qiime_results/cd_mc100/bdiv_even24476//chord_dm.txt Stdout: Stderr: # Principal coordinates (chord) command principal_coordinates.py -i qiime_results/cd_mc100/bdiv_even24476//chord_dm.txt -o qiime_results/cd_mc100/bdiv_even24476//chord_pc.txt Stdout: Stderr: # Make emperor plots, chord) command make_emperor.py -i qiime_results/cd_mc100/bdiv_even24476//chord_pc.txt -o qiime_results/cd_mc100/bdiv_even24476//chord_emperor_pcoa_plot/ -m meta.txt Stdout: Stderr: # Beta Diversity (euclidean) command beta_diversity.py -i qiime_results/cd_mc100/table_even24476.biom -o qiime_results/cd_mc100/bdiv_even24476/ --metrics euclidean -t qiime_results/data_mc100/rep_set_mc100.tre Stdout: Stderr: /usr/local/lib/python2.7/dist-packages/numpy/core/fromnumeric.py:2507: VisibleDeprecationWarning: `rank` is deprecated; use the `ndim` attribute or function instead. To find the rank of a matrix see `numpy.linalg.matrix_rank`. VisibleDeprecationWarning) # Rename distance matrix (euclidean) command mv qiime_results/cd_mc100/bdiv_even24476//euclidean_table_even24476.txt qiime_results/cd_mc100/bdiv_even24476//euclidean_dm.txt Stdout: Stderr: # Principal coordinates (euclidean) command principal_coordinates.py -i qiime_results/cd_mc100/bdiv_even24476//euclidean_dm.txt -o qiime_results/cd_mc100/bdiv_even24476//euclidean_pc.txt Stdout: Stderr: # Make emperor plots, euclidean) command make_emperor.py -i qiime_results/cd_mc100/bdiv_even24476//euclidean_pc.txt -o qiime_results/cd_mc100/bdiv_even24476//euclidean_emperor_pcoa_plot/ -m meta.txt Stdout: Stderr: # Beta Diversity (gower) command beta_diversity.py -i qiime_results/cd_mc100/table_even24476.biom -o qiime_results/cd_mc100/bdiv_even24476/ --metrics gower -t qiime_results/data_mc100/rep_set_mc100.tre Stdout: Stderr: /usr/local/lib/python2.7/dist-packages/numpy/core/fromnumeric.py:2507: VisibleDeprecationWarning: `rank` is deprecated; use the `ndim` attribute or function instead. To find the rank of a matrix see `numpy.linalg.matrix_rank`. VisibleDeprecationWarning) # Rename distance matrix (gower) command mv qiime_results/cd_mc100/bdiv_even24476//gower_table_even24476.txt qiime_results/cd_mc100/bdiv_even24476//gower_dm.txt Stdout: Stderr: # Principal coordinates (gower) command principal_coordinates.py -i qiime_results/cd_mc100/bdiv_even24476//gower_dm.txt -o qiime_results/cd_mc100/bdiv_even24476//gower_pc.txt Stdout: Stderr: /usr/local/lib/python2.7/dist-packages/skbio/stats/ordination/_principal_coordinate_analysis.py:107: RuntimeWarning: The result contains negative eigenvalues. Please compare their magnitude with the magnitude of some of the largest positive eigenvalues. If the negative ones are smaller, it's probably safe to ignore them, but if they are large in magnitude, the results won't be useful. See the Notes section for more details. The smallest eigenvalue is -5969.55591507 and the largest is 28387.1375242. RuntimeWarning # Make emperor plots, gower) command make_emperor.py -i qiime_results/cd_mc100/bdiv_even24476//gower_pc.txt -o qiime_results/cd_mc100/bdiv_even24476//gower_emperor_pcoa_plot/ -m meta.txt Stdout: Stderr: # Beta Diversity (hellinger) command beta_diversity.py -i qiime_results/cd_mc100/table_even24476.biom -o qiime_results/cd_mc100/bdiv_even24476/ --metrics hellinger -t qiime_results/data_mc100/rep_set_mc100.tre Stdout: Stderr: /usr/local/lib/python2.7/dist-packages/numpy/core/fromnumeric.py:2507: VisibleDeprecationWarning: `rank` is deprecated; use the `ndim` attribute or function instead. To find the rank of a matrix see `numpy.linalg.matrix_rank`. VisibleDeprecationWarning) # Rename distance matrix (hellinger) command mv qiime_results/cd_mc100/bdiv_even24476//hellinger_table_even24476.txt qiime_results/cd_mc100/bdiv_even24476//hellinger_dm.txt Stdout: Stderr: # Principal coordinates (hellinger) command principal_coordinates.py -i qiime_results/cd_mc100/bdiv_even24476//hellinger_dm.txt -o qiime_results/cd_mc100/bdiv_even24476//hellinger_pc.txt Stdout: Stderr: # Make emperor plots, hellinger) command make_emperor.py -i qiime_results/cd_mc100/bdiv_even24476//hellinger_pc.txt -o qiime_results/cd_mc100/bdiv_even24476//hellinger_emperor_pcoa_plot/ -m meta.txt Stdout: Stderr: # Beta Diversity (kulczynski) command beta_diversity.py -i qiime_results/cd_mc100/table_even24476.biom -o qiime_results/cd_mc100/bdiv_even24476/ --metrics kulczynski -t qiime_results/data_mc100/rep_set_mc100.tre Stdout: Stderr: /usr/local/lib/python2.7/dist-packages/numpy/core/fromnumeric.py:2507: VisibleDeprecationWarning: `rank` is deprecated; use the `ndim` attribute or function instead. To find the rank of a matrix see `numpy.linalg.matrix_rank`. VisibleDeprecationWarning) # Rename distance matrix (kulczynski) command mv qiime_results/cd_mc100/bdiv_even24476//kulczynski_table_even24476.txt qiime_results/cd_mc100/bdiv_even24476//kulczynski_dm.txt Stdout: Stderr: # Principal coordinates (kulczynski) command principal_coordinates.py -i qiime_results/cd_mc100/bdiv_even24476//kulczynski_dm.txt -o qiime_results/cd_mc100/bdiv_even24476//kulczynski_pc.txt Stdout: Stderr: /usr/local/lib/python2.7/dist-packages/skbio/stats/ordination/_principal_coordinate_analysis.py:107: RuntimeWarning: The result contains negative eigenvalues. Please compare their magnitude with the magnitude of some of the largest positive eigenvalues. If the negative ones are smaller, it's probably safe to ignore them, but if they are large in magnitude, the results won't be useful. See the Notes section for more details. The smallest eigenvalue is -0.0901120846473 and the largest is 3.41574508444. RuntimeWarning # Make emperor plots, kulczynski) command make_emperor.py -i qiime_results/cd_mc100/bdiv_even24476//kulczynski_pc.txt -o qiime_results/cd_mc100/bdiv_even24476//kulczynski_emperor_pcoa_plot/ -m meta.txt Stdout: Stderr: # Beta Diversity (manhattan) command beta_diversity.py -i qiime_results/cd_mc100/table_even24476.biom -o qiime_results/cd_mc100/bdiv_even24476/ --metrics manhattan -t qiime_results/data_mc100/rep_set_mc100.tre Stdout: Stderr: /usr/local/lib/python2.7/dist-packages/numpy/core/fromnumeric.py:2507: VisibleDeprecationWarning: `rank` is deprecated; use the `ndim` attribute or function instead. To find the rank of a matrix see `numpy.linalg.matrix_rank`. VisibleDeprecationWarning) # Rename distance matrix (manhattan) command mv qiime_results/cd_mc100/bdiv_even24476//manhattan_table_even24476.txt qiime_results/cd_mc100/bdiv_even24476//manhattan_dm.txt Stdout: Stderr: # Principal coordinates (manhattan) command principal_coordinates.py -i qiime_results/cd_mc100/bdiv_even24476//manhattan_dm.txt -o qiime_results/cd_mc100/bdiv_even24476//manhattan_pc.txt Stdout: Stderr: /usr/local/lib/python2.7/dist-packages/skbio/stats/ordination/_principal_coordinate_analysis.py:107: RuntimeWarning: The result contains negative eigenvalues. Please compare their magnitude with the magnitude of some of the largest positive eigenvalues. If the negative ones are smaller, it's probably safe to ignore them, but if they are large in magnitude, the results won't be useful. See the Notes section for more details. The smallest eigenvalue is -215935435.61 and the largest is 8185144152.74. RuntimeWarning # Make emperor plots, manhattan) command make_emperor.py -i qiime_results/cd_mc100/bdiv_even24476//manhattan_pc.txt -o qiime_results/cd_mc100/bdiv_even24476//manhattan_emperor_pcoa_plot/ -m meta.txt Stdout: Stderr: # Beta Diversity (morisita_horn) command beta_diversity.py -i qiime_results/cd_mc100/table_even24476.biom -o qiime_results/cd_mc100/bdiv_even24476/ --metrics morisita_horn -t qiime_results/data_mc100/rep_set_mc100.tre Stdout: Stderr: /usr/local/lib/python2.7/dist-packages/numpy/core/fromnumeric.py:2507: VisibleDeprecationWarning: `rank` is deprecated; use the `ndim` attribute or function instead. To find the rank of a matrix see `numpy.linalg.matrix_rank`. VisibleDeprecationWarning) # Rename distance matrix (morisita_horn) command mv qiime_results/cd_mc100/bdiv_even24476//morisita_horn_table_even24476.txt qiime_results/cd_mc100/bdiv_even24476//morisita_horn_dm.txt Stdout: Stderr: # Principal coordinates (morisita_horn) command principal_coordinates.py -i qiime_results/cd_mc100/bdiv_even24476//morisita_horn_dm.txt -o qiime_results/cd_mc100/bdiv_even24476//morisita_horn_pc.txt Stdout: Stderr: /usr/local/lib/python2.7/dist-packages/skbio/stats/ordination/_principal_coordinate_analysis.py:107: RuntimeWarning: The result contains negative eigenvalues. Please compare their magnitude with the magnitude of some of the largest positive eigenvalues. If the negative ones are smaller, it's probably safe to ignore them, but if they are large in magnitude, the results won't be useful. See the Notes section for more details. The smallest eigenvalue is -0.328252462089 and the largest is 5.30758161206. RuntimeWarning # Make emperor plots, morisita_horn) command make_emperor.py -i qiime_results/cd_mc100/bdiv_even24476//morisita_horn_pc.txt -o qiime_results/cd_mc100/bdiv_even24476//morisita_horn_emperor_pcoa_plot/ -m meta.txt Stdout: Stderr: # Beta Diversity (pearson) command beta_diversity.py -i qiime_results/cd_mc100/table_even24476.biom -o qiime_results/cd_mc100/bdiv_even24476/ --metrics pearson -t qiime_results/data_mc100/rep_set_mc100.tre Stdout: Stderr: /usr/local/lib/python2.7/dist-packages/numpy/core/fromnumeric.py:2507: VisibleDeprecationWarning: `rank` is deprecated; use the `ndim` attribute or function instead. To find the rank of a matrix see `numpy.linalg.matrix_rank`. VisibleDeprecationWarning) # Rename distance matrix (pearson) command mv qiime_results/cd_mc100/bdiv_even24476//pearson_table_even24476.txt qiime_results/cd_mc100/bdiv_even24476//pearson_dm.txt Stdout: Stderr: # Principal coordinates (pearson) command principal_coordinates.py -i qiime_results/cd_mc100/bdiv_even24476//pearson_dm.txt -o qiime_results/cd_mc100/bdiv_even24476//pearson_pc.txt Stdout: Stderr: /usr/local/lib/python2.7/dist-packages/skbio/stats/ordination/_principal_coordinate_analysis.py:107: RuntimeWarning: The result contains negative eigenvalues. Please compare their magnitude with the magnitude of some of the largest positive eigenvalues. If the negative ones are smaller, it's probably safe to ignore them, but if they are large in magnitude, the results won't be useful. See the Notes section for more details. The smallest eigenvalue is -0.373997044268 and the largest is 5.52789452742. RuntimeWarning # Make emperor plots, pearson) command make_emperor.py -i qiime_results/cd_mc100/bdiv_even24476//pearson_pc.txt -o qiime_results/cd_mc100/bdiv_even24476//pearson_emperor_pcoa_plot/ -m meta.txt Stdout: Stderr: # Beta Diversity (soergel) command beta_diversity.py -i qiime_results/cd_mc100/table_even24476.biom -o qiime_results/cd_mc100/bdiv_even24476/ --metrics soergel -t qiime_results/data_mc100/rep_set_mc100.tre Stdout: Stderr: /usr/local/lib/python2.7/dist-packages/numpy/core/fromnumeric.py:2507: VisibleDeprecationWarning: `rank` is deprecated; use the `ndim` attribute or function instead. To find the rank of a matrix see `numpy.linalg.matrix_rank`. VisibleDeprecationWarning) # Rename distance matrix (soergel) command mv qiime_results/cd_mc100/bdiv_even24476//soergel_table_even24476.txt qiime_results/cd_mc100/bdiv_even24476//soergel_dm.txt Stdout: Stderr: # Principal coordinates (soergel) command principal_coordinates.py -i qiime_results/cd_mc100/bdiv_even24476//soergel_dm.txt -o qiime_results/cd_mc100/bdiv_even24476//soergel_pc.txt Stdout: Stderr: # Make emperor plots, soergel) command make_emperor.py -i qiime_results/cd_mc100/bdiv_even24476//soergel_pc.txt -o qiime_results/cd_mc100/bdiv_even24476//soergel_emperor_pcoa_plot/ -m meta.txt Stdout: Stderr: # Beta Diversity (spearman_approx) command beta_diversity.py -i qiime_results/cd_mc100/table_even24476.biom -o qiime_results/cd_mc100/bdiv_even24476/ --metrics spearman_approx -t qiime_results/data_mc100/rep_set_mc100.tre Stdout: Stderr: /usr/local/lib/python2.7/dist-packages/numpy/core/fromnumeric.py:2507: VisibleDeprecationWarning: `rank` is deprecated; use the `ndim` attribute or function instead. To find the rank of a matrix see `numpy.linalg.matrix_rank`. VisibleDeprecationWarning) # Rename distance matrix (spearman_approx) command mv qiime_results/cd_mc100/bdiv_even24476//spearman_approx_table_even24476.txt qiime_results/cd_mc100/bdiv_even24476//spearman_approx_dm.txt Stdout: Stderr: # Principal coordinates (spearman_approx) command principal_coordinates.py -i qiime_results/cd_mc100/bdiv_even24476//spearman_approx_dm.txt -o qiime_results/cd_mc100/bdiv_even24476//spearman_approx_pc.txt Stdout: Stderr: /usr/local/lib/python2.7/dist-packages/skbio/stats/ordination/_principal_coordinate_analysis.py:107: RuntimeWarning: The result contains negative eigenvalues. Please compare their magnitude with the magnitude of some of the largest positive eigenvalues. If the negative ones are smaller, it's probably safe to ignore them, but if they are large in magnitude, the results won't be useful. See the Notes section for more details. The smallest eigenvalue is -0.0816464819489 and the largest is 1.57204571127. RuntimeWarning # Make emperor plots, spearman_approx) command make_emperor.py -i qiime_results/cd_mc100/bdiv_even24476//spearman_approx_pc.txt -o qiime_results/cd_mc100/bdiv_even24476//spearman_approx_emperor_pcoa_plot/ -m meta.txt Stdout: Stderr: # Beta Diversity (specprof) command beta_diversity.py -i qiime_results/cd_mc100/table_even24476.biom -o qiime_results/cd_mc100/bdiv_even24476/ --metrics specprof -t qiime_results/data_mc100/rep_set_mc100.tre Stdout: Stderr: /usr/local/lib/python2.7/dist-packages/numpy/core/fromnumeric.py:2507: VisibleDeprecationWarning: `rank` is deprecated; use the `ndim` attribute or function instead. To find the rank of a matrix see `numpy.linalg.matrix_rank`. VisibleDeprecationWarning) # Rename distance matrix (specprof) command mv qiime_results/cd_mc100/bdiv_even24476//specprof_table_even24476.txt qiime_results/cd_mc100/bdiv_even24476//specprof_dm.txt Stdout: Stderr: # Principal coordinates (specprof) command principal_coordinates.py -i qiime_results/cd_mc100/bdiv_even24476//specprof_dm.txt -o qiime_results/cd_mc100/bdiv_even24476//specprof_pc.txt Stdout: Stderr: # Make emperor plots, specprof) command make_emperor.py -i qiime_results/cd_mc100/bdiv_even24476//specprof_pc.txt -o qiime_results/cd_mc100/bdiv_even24476//specprof_emperor_pcoa_plot/ -m meta.txt Stdout: Stderr: # Beta Diversity (unifrac) command beta_diversity.py -i qiime_results/cd_mc100/table_even24476.biom -o qiime_results/cd_mc100/bdiv_even24476/ --metrics unifrac -t qiime_results/data_mc100/rep_set_mc100.tre Stdout: Stderr: # Rename distance matrix (unifrac) command mv qiime_results/cd_mc100/bdiv_even24476//unifrac_table_even24476.txt qiime_results/cd_mc100/bdiv_even24476//unifrac_dm.txt Stdout: Stderr: # Principal coordinates (unifrac) command principal_coordinates.py -i qiime_results/cd_mc100/bdiv_even24476//unifrac_dm.txt -o qiime_results/cd_mc100/bdiv_even24476//unifrac_pc.txt Stdout: Stderr: /usr/local/lib/python2.7/dist-packages/skbio/stats/ordination/_principal_coordinate_analysis.py:107: RuntimeWarning: The result contains negative eigenvalues. Please compare their magnitude with the magnitude of some of the largest positive eigenvalues. If the negative ones are smaller, it's probably safe to ignore them, but if they are large in magnitude, the results won't be useful. See the Notes section for more details. The smallest eigenvalue is -0.0276750462218 and the largest is 1.43139601061. RuntimeWarning # Make emperor plots, unifrac) command make_emperor.py -i qiime_results/cd_mc100/bdiv_even24476//unifrac_pc.txt -o qiime_results/cd_mc100/bdiv_even24476//unifrac_emperor_pcoa_plot/ -m meta.txt Stdout: Stderr: # Beta Diversity (unweighted_unifrac) command beta_diversity.py -i qiime_results/cd_mc100/table_even24476.biom -o qiime_results/cd_mc100/bdiv_even24476/ --metrics unweighted_unifrac -t qiime_results/data_mc100/rep_set_mc100.tre Stdout: Stderr: # Rename distance matrix (unweighted_unifrac) command mv qiime_results/cd_mc100/bdiv_even24476//unweighted_unifrac_table_even24476.txt qiime_results/cd_mc100/bdiv_even24476//unweighted_unifrac_dm.txt Stdout: Stderr: # Principal coordinates (unweighted_unifrac) command principal_coordinates.py -i qiime_results/cd_mc100/bdiv_even24476//unweighted_unifrac_dm.txt -o qiime_results/cd_mc100/bdiv_even24476//unweighted_unifrac_pc.txt Stdout: Stderr: /usr/local/lib/python2.7/dist-packages/skbio/stats/ordination/_principal_coordinate_analysis.py:107: RuntimeWarning: The result contains negative eigenvalues. Please compare their magnitude with the magnitude of some of the largest positive eigenvalues. If the negative ones are smaller, it's probably safe to ignore them, but if they are large in magnitude, the results won't be useful. See the Notes section for more details. The smallest eigenvalue is -0.0276750462218 and the largest is 1.43139601061. RuntimeWarning # Make emperor plots, unweighted_unifrac) command make_emperor.py -i qiime_results/cd_mc100/bdiv_even24476//unweighted_unifrac_pc.txt -o qiime_results/cd_mc100/bdiv_even24476//unweighted_unifrac_emperor_pcoa_plot/ -m meta.txt Stdout: Stderr: # Beta Diversity (unweighted_unifrac_full_tree) command beta_diversity.py -i qiime_results/cd_mc100/table_even24476.biom -o qiime_results/cd_mc100/bdiv_even24476/ --metrics unweighted_unifrac_full_tree -t qiime_results/data_mc100/rep_set_mc100.tre Stdout: Stderr: # Rename distance matrix (unweighted_unifrac_full_tree) command mv qiime_results/cd_mc100/bdiv_even24476//unweighted_unifrac_full_tree_table_even24476.txt qiime_results/cd_mc100/bdiv_even24476//unweighted_unifrac_full_tree_dm.txt Stdout: Stderr: # Principal coordinates (unweighted_unifrac_full_tree) command principal_coordinates.py -i qiime_results/cd_mc100/bdiv_even24476//unweighted_unifrac_full_tree_dm.txt -o qiime_results/cd_mc100/bdiv_even24476//unweighted_unifrac_full_tree_pc.txt Stdout: Stderr: /usr/local/lib/python2.7/dist-packages/skbio/stats/ordination/_principal_coordinate_analysis.py:107: RuntimeWarning: The result contains negative eigenvalues. Please compare their magnitude with the magnitude of some of the largest positive eigenvalues. If the negative ones are smaller, it's probably safe to ignore them, but if they are large in magnitude, the results won't be useful. See the Notes section for more details. The smallest eigenvalue is -0.0379791808386 and the largest is 0.86720141835. RuntimeWarning # Make emperor plots, unweighted_unifrac_full_tree) command make_emperor.py -i qiime_results/cd_mc100/bdiv_even24476//unweighted_unifrac_full_tree_pc.txt -o qiime_results/cd_mc100/bdiv_even24476//unweighted_unifrac_full_tree_emperor_pcoa_plot/ -m meta.txt Stdout: Stderr: # Beta Diversity (weighted_normalized_unifrac) command beta_diversity.py -i qiime_results/cd_mc100/table_even24476.biom -o qiime_results/cd_mc100/bdiv_even24476/ --metrics weighted_normalized_unifrac -t qiime_results/data_mc100/rep_set_mc100.tre Stdout: Stderr: # Rename distance matrix (weighted_normalized_unifrac) command mv qiime_results/cd_mc100/bdiv_even24476//weighted_normalized_unifrac_table_even24476.txt qiime_results/cd_mc100/bdiv_even24476//weighted_normalized_unifrac_dm.txt Stdout: Stderr: # Principal coordinates (weighted_normalized_unifrac) command principal_coordinates.py -i qiime_results/cd_mc100/bdiv_even24476//weighted_normalized_unifrac_dm.txt -o qiime_results/cd_mc100/bdiv_even24476//weighted_normalized_unifrac_pc.txt Stdout: Stderr: /usr/local/lib/python2.7/dist-packages/skbio/stats/ordination/_principal_coordinate_analysis.py:107: RuntimeWarning: The result contains negative eigenvalues. Please compare their magnitude with the magnitude of some of the largest positive eigenvalues. If the negative ones are smaller, it's probably safe to ignore them, but if they are large in magnitude, the results won't be useful. See the Notes section for more details. The smallest eigenvalue is -0.0571324096723 and the largest is 3.81299497897. RuntimeWarning # Make emperor plots, weighted_normalized_unifrac) command make_emperor.py -i qiime_results/cd_mc100/bdiv_even24476//weighted_normalized_unifrac_pc.txt -o qiime_results/cd_mc100/bdiv_even24476//weighted_normalized_unifrac_emperor_pcoa_plot/ -m meta.txt Stdout: Stderr: # Beta Diversity (weighted_unifrac) command beta_diversity.py -i qiime_results/cd_mc100/table_even24476.biom -o qiime_results/cd_mc100/bdiv_even24476/ --metrics weighted_unifrac -t qiime_results/data_mc100/rep_set_mc100.tre Stdout: Stderr: # Rename distance matrix (weighted_unifrac) command mv qiime_results/cd_mc100/bdiv_even24476//weighted_unifrac_table_even24476.txt qiime_results/cd_mc100/bdiv_even24476//weighted_unifrac_dm.txt Stdout: Stderr: # Principal coordinates (weighted_unifrac) command principal_coordinates.py -i qiime_results/cd_mc100/bdiv_even24476//weighted_unifrac_dm.txt -o qiime_results/cd_mc100/bdiv_even24476//weighted_unifrac_pc.txt Stdout: Stderr: /usr/local/lib/python2.7/dist-packages/skbio/stats/ordination/_principal_coordinate_analysis.py:107: RuntimeWarning: The result contains negative eigenvalues. Please compare their magnitude with the magnitude of some of the largest positive eigenvalues. If the negative ones are smaller, it's probably safe to ignore them, but if they are large in magnitude, the results won't be useful. See the Notes section for more details. The smallest eigenvalue is -0.0885259043797 and the largest is 2.61929477593. RuntimeWarning # Make emperor plots, weighted_unifrac) command make_emperor.py -i qiime_results/cd_mc100/bdiv_even24476//weighted_unifrac_pc.txt -o qiime_results/cd_mc100/bdiv_even24476//weighted_unifrac_emperor_pcoa_plot/ -m meta.txt Stdout: Stderr: Executing commands. # Alpha rarefaction command multiple_rarefactions.py -i qiime_results/cd_mc100/table_mc24476.biom -m 10 -x 24476 -s 2446 -o qiime_results/cd_mc100/arare_max24476//rarefaction/ Stdout: Stderr: # Alpha diversity on rarefied OTU tables command alpha_diversity.py -i qiime_results/cd_mc100/arare_max24476//rarefaction/ -o qiime_results/cd_mc100/arare_max24476//alpha_div/ --metrics berger_parker_d,brillouin_d,chao1,chao1_ci,dominance,doubles,enspie,equitability,esty_ci,fisher_alpha,gini_index,goods_coverage,heip_e,kempton_taylor_q,margalef,mcintosh_d,mcintosh_e,menhinick,observed_otus,observed_species,osd,simpson_reciprocal,robbins,shannon,simpson,simpson_e,singles,strong,PD_whole_tree -t qiime_results/data_mc100/rep_set_mc100.tre Stdout: Stderr: # Collate alpha command collate_alpha.py -i qiime_results/cd_mc100/arare_max24476//alpha_div/ -o qiime_results/cd_mc100/arare_max24476//alpha_div_collated/ Stdout: Stderr: # Removing intermediate files command rm -r qiime_results/cd_mc100/arare_max24476//rarefaction/ qiime_results/cd_mc100/arare_max24476//alpha_div/ Stdout: Stderr: # Rarefaction plot: All metrics command make_rarefaction_plots.py -i qiime_results/cd_mc100/arare_max24476//alpha_div_collated/ -m meta.txt -o qiime_results/cd_mc100/arare_max24476//alpha_rarefaction_plots/ Stdout: Stderr: /usr/local/lib/python2.7/dist-packages/matplotlib/axes/_base.py:2809: UserWarning: Attempting to set identical bottom==top results in singular transformations; automatically expanding. bottom=0, top=0 'bottom=%s, top=%s') % (bottom, top)) Skipping compare_alpha_diversity.py as no categories were provided. Executing commands. # Sort OTU Table command sort_otu_table.py -i qiime_results/cd_mc100/table_mc24476.biom -o qiime_results/cd_mc100/taxa_plots/table_mc24476_sorted.biom Stdout: Stderr: # Summarize Taxonomy command summarize_taxa.py -i qiime_results/cd_mc100/taxa_plots/table_mc24476_sorted.biom -o qiime_results/cd_mc100/taxa_plots/ --level 2,3,4,5,6,7 Stdout: Stderr: # Plot Taxonomy Summary command plot_taxa_summary.py -i qiime_results/cd_mc100/taxa_plots/table_mc24476_sorted_L2.txt,qiime_results/cd_mc100/taxa_plots/table_mc24476_sorted_L3.txt,qiime_results/cd_mc100/taxa_plots/table_mc24476_sorted_L4.txt,qiime_results/cd_mc100/taxa_plots/table_mc24476_sorted_L5.txt,qiime_results/cd_mc100/taxa_plots/table_mc24476_sorted_L6.txt,qiime_results/cd_mc100/taxa_plots/table_mc24476_sorted_L7.txt -o qiime_results/cd_mc100/taxa_plots//taxa_summary_plots/ --labels Phylum,Class,Order,Family,Genus,Species --chart_type bar,area,pie Stdout: Stderr: Executing commands. # Compress the filtered BIOM table command gzip qiime_results/cd_mc100/table_mc24476.biom Stdout: Stderr: # Compress the rarefied BIOM table command gzip qiime_results/cd_mc100/table_even24476.biom Stdout: Stderr: Logging stopped at 18:09:57 on 12 Oct 2016