Forsyth Bioinformatics

BLAST-Based Open-Reference 16S sRNA NGS Species-Level Read Assignment Pipeline With QIIME Results Code Category Minimal read count per species (MC) (Click for detail report) MC1 (All) MC2 MC5 MC10 MC50 MC100 A Total reads 13,431,314 13,431,314 13,431,314 13,431,314 13,431,314 13,431,314 B Total samples 77 77 77 77 77 77 C Samples with reads ≥ 500 reads (S≥500) 77 77 77 77 77 77 D Samples with reads < 500 reads (S<500) 0 0 0 0 0 0 E Reads in samples S≥500 13,431,314 13,431,314 13,431,314 13,431,314 13,431,314 13,431,314 F Reads in samples S<500 (removed) 0 0 0 0 0 0 G Total reads assigned 12,938,187 12,938,060 12,934,253 12,930,782 12,912,691 12,898,490 H Reads assigned to single species 9,513,214 9,513,087 9,512,609 9,511,993 9,507,524 9,503,566 I Reads assigned to multiple species 2,766,141 2,766,141 2,765,809 2,765,362 2,762,385 2,759,073 J Reads assigned to novel species 658,832 658,832 655,835 653,427 642,782 635,851 K Number of single species 1,001 874 701 609 420 363 L Number of multi-species 505 505 376 309 186 141 M Number of novel species 2,269 2,269 1,114 747 268 170 N Total reads unassigned 493,127 493,254 497,061 500,532 518,623 532,824 O Reads excluded (<MC) 0 127 3,934 7,405 25,496 39,697 P Chimeric reads 187,396 187,396 187,396 187,396 187,396 187,396 Q Reads without BLASTN hits 921 921 921 921 921 921 R Singleton reads 304,810 304,810 304,810 304,810 304,810 304,810 1. A=E+F A=F+G+N G=H+I+J N=O+P+Q+R 2. View all read count by samples 3. All data can be downloaded in a compressed ZIP file ZIP file 4. All data are also available on the FTP site for 30 days 5. Please read DOCUMENTATION (pdf) 6. Preferred web browser: Google Chrome or FireFox, especially for 2D and 3D plots 7. Contact: George Chen, Email: tchen\@forsyth.org for questions